Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level

Despite recent advances in spatial transcriptomics to map gene expression, it has not be possible to determine the underlying epigenetic mechanisms controlling gene expression and tissue development with high spatial resolution. Deng et al. report on a technique called spatial-CUT&Tag for genome-wide profiling of histone modifications pixel by pixel on a frozen tissue section without dissociation. This method resolved spatially distinct and cell-type-specific chromatin modifications in mouse embryonic organogenesis and postnatal brain development. Single-cell epigenomic profiles were derived from the tissue pixels containing single nuclei. Spatial-CUT&Tag adds a new dimension to spatial biology by enabling the mapping of epigenetic regulations broadly implicated in development and disease.

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